PCR Equipment and Supplies
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Applied Biosystems™ GeneAmp™ 10X PCR Buffer
Applied Biosystems GeneAmp 10X PCR Buffer I is optimized for use with AmpliTaq DNA Polymerase, resulting in robust PCR amplification.
Non-distribution item offered as a customer accommodation; additional freight charges may apply.
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| Chemical Name or Material | PCR Buffer |
|---|---|
| Concentration | 10X |
| For Use With (Application) | Standard PCR |
Thermo Scientific™ dCTP Solution (100 mM)
Thermo Scientific dCTP (2'-deoxycytidine 5'-triphosphate) is highly stable nucleotide, supplied as 100 mM aqueous solution titrated to pH 7.3-7.5 with NaOH.
Applied Biosystems™ Absolute Q™ 1-step RT-dPCR Master Mix (4X)
Applied Biosystems Absolute Q 1-step RT-dPCR Master Mix (4X) is optimized for use with the QuantStudio Absolute Q Digital PCR System in a simple workflow with minimal processing steps.
Non-distribution item offered as a customer accommodation; additional freight charges may apply.
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Thermo Scientific™ Mutation Generation System Kit
Perform functional analysis of proteins with these transposon-based tools.
Invitrogen™ Cells-to-cDNA™ II Kit
The Ambion™ Cells-to-cDNA™ II Kit (patent pending) produces cDNA from cultured mammalian cells in less than 2 hr., no RNA isolation is required.
Non-distribution item offered as a customer accommodation; additional freight charges may apply.
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| Shipping Condition | Dry Ice |
|---|---|
| Content And Storage | 1X PBS, Cell Lysis II Buffer, μL DNase 1, 10X RT Buffer, M-mLV Reverse Transcriptase, RNase Inhibitor, dNTP Mix, Random Decamers, Oligo(dT)18 Primers, Armored RNA™ Control, Armored RNA™ Primer Pair, and Endogenous Primer Pair should be stored at -20°C. Nuclease-free water may be stored at any temperature. |
| Reverse Transcriptase | M-MLV |
| Size (Final Product) | 7 kb or less |
| Final Product Type | cDNA |
| Sample Type | Mammalian Cells |
| Purification Time | 2 hr. |
| Optimal Reaction Temperature | 42°C |
| For Use With (Application) | Real Time PCR (qPCR),RT-PCR |
| Product Line | Ambion, Cells-to-cDNA |
Applied Biosystems™ AmpliTaq™ DNA Polymerase with Buffer I or Buffer II
AmpliTaq DNA Polymerase is a 94 kDa, thermostable, recombinant DNA polymerase obtained by expression of a modified form of the Thermus aquaticus (Taq) DNA polymerase gene in E. coli.
Non-distribution item offered as a customer accommodation; additional freight charges may apply.
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| Shipping Condition | Dry Ice |
|---|---|
| Fidelity (vs. Taq) | 1X |
| Overhang | 3'-A |
| Concentration | 5 U/μL |
| For Use With (Application) | Standard PCR |
| Exonuclease Activity | 5' - 3' |
| Polymerase | AmpliTaq DNA Polymerase |
| Format | Stand-alone enzyme |
| Size (Final Product) | 5 kb or less |
| Reaction Format | Separate Components |
| Product Type | DNA Polymerase |
| Reaction Speed | Standard |
| Hot Start | No |
| Starting Material | DNA |
Thermo Scientific™ Taq DNA Polymerase, native (5 U/μL)
Thermo Scientific Native Taq DNA Polymerase is a highly thermostable DNA polymerase of the thermophilic bacterium Thermus aquaticus.
| Polymerase | Taq DNA Polymerase |
|---|---|
| Product Type | DNA Polymerase |
| Concentration | 5 U/μL |
| For Use With (Application) | Standard PCR |
Applied Biosystems™ TaqPath™ 1-Step Multiplex Master Mix
TaqPath 1-Step Multiplex Master Mix is a fast, single-tube, 4X RT-qPCR mix that provides for sensitive, reproducible detection of up to four different RNA/DNA targets in a single multiplex reaction.
Non-distribution item offered as a customer accommodation; additional freight charges may apply.
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| Shipping Condition | Dry Ice |
|---|---|
| InhibitorToleranceSimple | High |
| Concentration | 4X |
| For Use With (Application) | Gene Expression,Microbial Detection |
| PCR Method | 1-step RT-qPCR |
| Multiplex Capability | Up to 4-plex |
| Detection Method | Primer-probe |
| Includes | dUTP, UNG/UDG |
| Thermostability | Not Applicable |
| Polymerase | Fast DNA Polymerase |
| Sensitivity | Down to 10 copies |
| Format | Tube |
| Product Type | qPCR Master Mix |
| Passive Reference Dye | Mustang Purple |
| For Use With (Equipment) | QuantStudio 6 Pro Real-Time PCR System or QuantStudio 7 Pro, QuantStudio 3 or 5, QuantStudio 6/QuantStudio 7 Flex, QuantStudio 12K Flex, QuantStudio Dx, ViiA 7, 7500 , 7500 Fast, 7500 Fast Dx |
| Sample Type | DNA, RNA |
| Reaction Speed | Fast or Standard |
| Target Specificity | RNA only, Both RNA and DNA |
| Product Line | TaqPath |
Applied Biosystems™ Oligo d(T)16 (50 μM)
Invitrogen Oligo(dT)16 Primer is a homo-oligomeric deoxyribonucleotide poly(dT) that is non-phosphorylated and chemically synthesized.
Non-distribution item offered as a customer accommodation; additional freight charges may apply.
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Invitrogen™ ChargeSwitch™ PCR Clean-Up Kit
ChargeSwitch PCR Clean-Up Kit is the fastest and most effective method of removing primers, unincorporated dNTPs, thermostable polymerases and salts.
Non-distribution item offered as a customer accommodation; additional freight charges may apply.
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| Shipping Condition | Room Temperature |
|---|---|
| Target | DNA from Enzymatic Reactions (e.g. PCR) |
| Content And Storage | The ChargeSwitch™ PCR Clean-Up Kit is shipped at room temperature. Upon receipt, store all components at room temperature. All components are guaranteed stable for 6 months when stored properly. |
| Format | Kit |
| Product Type | PCR Clean-Up Kit |
| Sample Type | PCR Products |
| Isolation Technology | ChargeSwitch™ Chemistry |
| Run Time | 5 min |
| For Use With (Application) | Next-Generation Sequencing (NGS),Cloning,Standard PCR |
| High-throughput Compatibility | High-throughput Compatible |
| Product Line | ChargeSwitch |
Invitrogen™ Bio-11-UTP (10 mM)
Ambion Bio-11-UTP 10 mM (Biotinylated UTP) is intended for use as substrate in in vitro transcription reactions.
Non-distribution item offered as a customer accommodation; additional freight charges may apply.
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Invitrogen™ Template Switching Reverse Transcription Oligonucleotides
Use this set of oligonucleotides for template switching cDNA synthesis and preamplification. Template Switching Oligonucleotide and Capturing Oligo (dT) are used to incorporate adaptor sequences to full-length cDNA. Preamplification primer enables PCR amplification of obtained cDNA.
Non-distribution item offered as a customer accommodation; additional freight charges may apply.
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| Color | Colorless |
|---|---|
| Label or Dye | No |
| Quantity | 48 Reactions |
Invitrogen™ PureLink™ PCR Micro Kit
PureLink PCR Micro Kits are ideal for purifying and concentrating DNA from low-yielding PCR or restriction digestion reactions.
Non-distribution item offered as a customer accommodation; additional freight charges may apply.
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| Shipping Condition | Room Temperature |
|---|---|
| Separation Range | 100 bp to 12 kb |
| Content And Storage | Kit includes columns, binding and wash buffers, recovery tubes, and the manual. Store at room temperature. |
| Starting Material Volume | 125 μL |
| Isolation Technology | Silica Spin Column |
| Elution Volume Max | 20 μL |
| For Use With (Application) | Cloning,Standard PCR |
| High-throughput Compatibility | Not High-throughput Compatible (Manual) |
| Target | DNA from Enzymatic Reactions (e.g. PCR) |
| Format | Spin Column |
| Product Type | PCR Micro Kit |
| Run Time | 10 min. |
| Elution Volume Min | 5 μL |
| Product Line | PureLink |
Applied Biosystems™ AmpliTaq Gold™ DNA Polymerase with Buffer I
AmpliTaq Gold DNA Polymerase is a chemically modified form of AmpliTaq DNA Polymerase requiring thermal activation.
Non-distribution item offered as a customer accommodation; additional freight charges may apply.
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| GC-Rich PCR Performance | Low |
|---|---|
| Fidelity (vs. Taq) | 1X |
| Product Type | DNA Polymerase With Buffer I |
| Hot Start | Built-In Hot Start |
| Overhang | 3'-A |
| Concentration | 5 U/μL |
| For Use With (Application) | Hot-start PCR |
| Exonuclease Activity | 5' - 3' |
Thermo Scientific™ Exo-Resistant Random Primer
Thermo Scientific Exonuclease-Resistant Random Primer is a mixture of single-stranded random oligonucleotides used for highly efficient random priming of various DNA synthesis reactions.