PCR Equipment and Supplies
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Applied Biosystems™ Oligo d(T)16 (50 μM)
Invitrogen Oligo(dT)16 Primer is a homo-oligomeric deoxyribonucleotide poly(dT) that is non-phosphorylated and chemically synthesized.
Non-distribution item offered as a customer accommodation; additional freight charges may apply.
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Thermo Scientific™ Maxima Reverse Transcriptase (200 U/μL)
Thermo Scientific Maxima Reverse Transcriptase (RT) was developed through in vitro evolution of M-MuLV RT. The enzyme possesses an RNA-dependent and DNA-dependent polymerase activity as well as RNase H activity.
| Concentration | 200 U/μL |
|---|
Applied Biosystems™ VetMAX™ PRRSV 3.0 Controls
VetMAX™ PRRSV 3.0 Controls are for use in multiplex, real-time reverse transcription PCR (RT-PCR) assays targeting North American and European porcine reproductive and respiratory syndrome virus (PRRSV) and Xeno RNA.
Non-distribution item offered as a customer accommodation; additional freight charges may apply.
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Applied Biosystems™ Power SYBR™ Green RNA-to-CT™ 1-Step Kit
The novel formulation reduces false positive results caused by primer dimers, significantly improves data accuracy, and provides reliable detection of low abundance targets.
Non-distribution item offered as a customer accommodation; additional freight charges may apply.
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| Shipping Condition | Wet Ice |
|---|---|
| Purity or Quality Grade | UP (Ultra Pure) |
| Reverse Transcriptase | ArrayScript™ UP |
| For Use With (Application) | Gene Expression |
| PCR Method | 1-step RT-qPCR |
| Detection Method | SYBR |
| Polymerase | AmpliTaq Gold DNA Polymerase |
| Passive Reference Dye | ROX (Pre-mixed) |
| For Use With (Equipment) | 7500 Fast System, 7500 System, 7900HT System, QuantStudio™ 12k Flex, QuantStudio™ 3, QuantStudio™ 5, QuantStudio™ 6 Flex, QuantStudio™ 7, StepOne™, StepOnePlus™, ViiA™ 7 System |
| Sample Type | RNA |
| Reaction Speed | Standard |
| Label or Dye | SYBR Green |
| Run Time | Standard |
| Product Line | RNA-to-CT, SYBR |
TaqMan™ PGx Express Panel, 384-well plate
This TaqMan PGx (Pharmacogenomics) Express Panel is offered in pre-spotted 384-well plates, an efficient, easy-to-use format for pharmacogenomics applications.
Non-distribution item offered as a customer accommodation; additional freight charges may apply.
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Invitrogen™ SuperScript™ IV Template Switching RT Master Mix
Invitrogen SuperScript IV Template-Switching RT Master Mix is designed for high template switching efficiency in cDNA synthesis reactions.
Non-distribution item offered as a customer accommodation; additional freight charges may apply.
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| GC-Rich PCR Performance | High |
|---|---|
| Format | Master Mix |
| Final Product Type | First-Strand cDNA |
| Reaction Speed | 30 min. |
| Reagent Type | Reverse Transcription |
| Technique | Template Switching Reverse Transcription |
| Concentration | 4X |
| Starting Material | Cells or RNA |
Thermo Scientific™ RevertAid H Minus First Strand cDNA Synthesis Kit
Thermo Scientific RevertAid H Minus First Strand cDNA Synthesis Kit is a complete system for efficient synthesis of first strand cDNA from RNA templates.
| Shipping Condition | Dry Ice |
|---|---|
| Format | Kit |
| Reverse Transcriptase | RevertAid H Minus |
| Size (Final Product) | Up to 13 kb |
| Reaction Format | Separate components |
| Final Product Type | First-Strand cDNA |
| Reaction Speed | 60 min. |
| Reagent Type | Reverse Transcription |
| Ribonuclease H Activity | Reduced |
| Optimal Reaction Temperature | 42°C to 45°C |
| Technique | Reverse Transcription |
| Starting Material | RNA |
| For Use With (Application) | Real Time PCR (qPCR),RT-PCR |
Thermo Scientific™ DreamTaq™ Hot Start DNA Polymerase
Thermo Scientific DreamTaq Hot Start DNA Polymerase is an enhanced hot start Taq DNA polymerase that enables higher PCR specificity, sensitivity, and yields compared to conventional hot start Taq DNA polymerases.
| Polymerase | DreamTaq DNA Polymerase |
|---|---|
| GC-Rich PCR Performance | Low |
| Reaction Format | Separate Components |
| Reaction Speed | Standard |
| Hot Start | Built-In Hot Start |
| Overhang | 3'-A |
| Concentration | 5 U/μL |
| For Use With (Application) | Hot-start PCR |
Invitrogen™ PureLink™ PCR Micro Kit
PureLink PCR Micro Kits are ideal for purifying and concentrating DNA from low-yielding PCR or restriction digestion reactions.
Non-distribution item offered as a customer accommodation; additional freight charges may apply.
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| Shipping Condition | Room Temperature |
|---|---|
| Separation Range | 100 bp to 12 kb |
| Content And Storage | Kit includes columns, binding and wash buffers, recovery tubes, and the manual. Store at room temperature. |
| Starting Material Volume | 125 μL |
| Isolation Technology | Silica Spin Column |
| Elution Volume Max | 20 μL |
| For Use With (Application) | Cloning,Standard PCR |
| High-throughput Compatibility | Not High-throughput Compatible (Manual) |
| Target | DNA from Enzymatic Reactions (e.g. PCR) |
| Format | Spin Column |
| Product Type | PCR Micro Kit |
| Run Time | 10 min. |
| Elution Volume Min | 5 μL |
| Product Line | PureLink |
Thermo Scientific™ dGTP Solution (100 mM)
Thermo Scientific dGTP (2'-deoxyguanosine 5'-triphosphate) is highly stable nucleotide, supplied as 100 mM aqueous solution titrated to pH 7.3-7.5 with NaOH.
Applied Biosystems™ TaqPath™ BactoPure™ Microbial Detection Master Mix
TaqPath BactoPure Microbial Detection Master Mix is optimized for rapid low-level microbial detection even in the presence of PCR inhibitors.
Non-distribution item offered as a customer accommodation; additional freight charges may apply.
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| Content And Storage | Contains 2X TaqPath BactoPure Microbial Detection Master Mix that includes: • Hot-start AmpliTaq Fast DNA Polymerase • Heat-labile uracil-DNA glycosylase (UNG) • dNTPs (with dUTP) • ROX dye (passive reference) • Optimized buffer Store at 2°C to 8°C in the dark. |
|---|---|
| InhibitorToleranceSimple | Very High |
| For Use With (Application) | Microbial Detection |
| PCR Method | qPCR |
| Multiplex Capability | Up to 3-plex |
| Detection Method | Primer-probe |
| Includes | dUTP, UNG/UDG |
| Thermostability | 72 hr., Assembled Reaction |
| Sensitivity | Down to 1 copy |
| Passive Reference Dye | ROX (Pre-mixed) |
| For Use With (Equipment) | 7500 Fast System, 7500 System, 7900HT Fast System, 7900HT System, QuantStudio12k Flex Dx, QuantStudio™ 12k Flex, QuantStudio™ 3, QuantStudio™ 5, QuantStudio 5 Dx, QuantStudio 6 Pro, QuantStudio™ 6 Flex, QuantStudio™ 7 Flex, QuantStudio 7 Pro, QuantStudio 7 Pro Dx, ViiA™ 7 System, ViiA 7 Dx, QuantStudio™ Dx, StepOnePlus™ System, 7500 Fast Dx System, StepOne™ System |
| Sample Type | DNA |
| Reaction Speed | Fast or Standard |
| Target Specificity | cDNA or gDNA only |
| Product Line | TaqPath |
Invitrogen™ Poly(A) Tailing Kit
For the polyadenylation of in vitro transcribed RNA to enhance translation initiation efficiency.
Non-distribution item offered as a customer accommodation; additional freight charges may apply.
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Thermo Scientific™ Taq DNA Polymerase, recombinant (1 U/μL)
Thermo Scientific Taq DNA Polymerase is a highly thermostable DNA polymerase from the thermophilic bacterium Thermus aquaticus.
Thermo Scientific™ dNTP Mix (2 mM each)
Thermo Scientific dNTP Mix (2 mM) contains premixed aqueous solutions of dATP, dCTP, dGTP and dTTP, each at a final concentration of 2 mM.
| Shipping Condition | Dry Ice |
|---|---|
| Purity or Quality Grade | >99% |
| Form | Liquid |
| Label or Dye | Unlabeled |
| Concentration | 2 mM |
| For Use With (Application) | Standard PCR,High-fidelity PCR,Real Time PCR (qPCR) |
Thermo Scientific™ Exo-Resistant Random Primer
Thermo Scientific Exonuclease-Resistant Random Primer is a mixture of single-stranded random oligonucleotides used for highly efficient random priming of various DNA synthesis reactions.