 
                                   PCR Equipment and Supplies
 
                                                 
                                                 
                                                 
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                                                                    Applied Biosystems™ SYBR™ Green Universal Master Mix
                                                            
                                                            
                                                            
                                                                
                                                            
                                                                
                                                            
                                        
                                                            
                                                            Everything needed for SYBR™ Green dye-based PCR amplification and detection in a convenient, single-tube format.
 
                                                                            
                                                                                
                                                                            
                                                                    
                                                                    
                                                                         
                                                                            
                                                                                Non-distribution item offered as a customer accommodation; additional freight charges may apply.
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| Polymerase | AmpliTaq Gold DNA Polymerase | 
|---|---|
| GC-Rich PCR Performance | High | 
| Format | Tube | 
| Product Type | Real Time PCR SYBR Master Mix | 
| Passive Reference Dye | ROX (Pre-mixed) | 
| Reaction Speed | Standard | 
| Label or Dye | SYBR Green | 
| Concentration | 2X | 
| For Use With (Application) | Gene Expression | 
| PCR Method | qPCR | 
| Product Line | SYBR™ | 
| Detection Method | SYBR | 
                                                                    Thermo Scientific™ Phusion Blood Direct PCR Kit
                                                            
                                                            
                                                            
                                                                
                                                            
                                                                
                                                            
                                        
                                                            
                                                            Thermo Scientific Phusion Blood Direct PCR Kit is developed for amplification of DNA from whole blood. It eliminates the need for a separate DNA purification step prior to PCR.
 
                                                                            
                                                                                
                                                                            
                                                                    
                                                                | Shipping Condition | Wet or Dry Ice | 
|---|---|
| Polymerase | Phusion Blood II DNA Polymerase | 
| GC-Rich PCR Performance | High | 
| Reaction Format | Separate Components | 
| Product Type | Direct PCR Kit | 
| Sample Type | Blood | 
| Reaction Speed | Fast | 
| Hot Start | Built-In Hot Start | 
| Overhang | Blunt | 
| Concentration | 2 U/μL | 
| Starting Material | DNA | 
| PCR Method | Direct PCR | 
                                                                    Invitrogen™ Platinum™ Taq Green Hot Start DNA Polymerase
                                                            
                                                            
                                                            
                                                                
                                                            
                                                                
                                                            
                                        
                                                            
                                                            Invitrogen Platinum Taq Green Hot Start DNA Polymerase provides Platinum Taq DNA Polymerase with a 10X Green PCR Buffer.
 
                                                                            
                                                                                
                                                                            
                                                                    
                                                                    
                                                                         
                                                                            
                                                                                Non-distribution item offered as a customer accommodation; additional freight charges may apply.
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                                                                    Applied Biosystems™ High-Capacity RNA-to-cDNA™ Kit
                                                            
                                                            
                                                            
                                                                
                                                            
                                                                
                                                            
                                        
                                                            
                                                            The High Capacity RNA-to-cDNA Kit is a streamlined reverse transcription kit designed for optimum performance with TaqMan Gene Expression Master Mix, Power SYBR Green PCR Master Mix, and other PCR enzymes.
 
                                                                            
                                                                                
                                                                            
                                                                    
                                                                    
                                                                         
                                                                            
                                                                                Non-distribution item offered as a customer accommodation; additional freight charges may apply.
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| Shipping Condition | Dry Ice | 
|---|---|
| Format | Kit | 
| Reverse Transcriptase | MultiScribe | 
| Size (Final Product) | Up to 7 kb | 
| Reaction Format | Premixed Components | 
| Final Product Type | First-Strand cDNA | 
| Reaction Speed | 30 to 60 min. | 
| Reagent Type | Reverse Transcription | 
| Ribonuclease H Activity | Yes | 
| Optimal Reaction Temperature | 37°C | 
| Technique | Reverse Transcription | 
| Starting Material | RNA | 
| For Use With (Application) | Real Time PCR (qPCR),RT-PCR | 
                                                                    Invitrogen™ Platinum™ Hot Start PCR Master Mixes (2X)
                                                            
                                                            
                                                            
                                                                
                                                            
                                                                
                                                            
                                        
                                                            
                                                            Invitrogen Platinum Hot Start PCR Master Mix offers Platinum Taq DNA polymerase in an optimized PCR buffer with magnesium and dNTPs for convenient PCR setup.
 
                                                                            
                                                                                
                                                                            
                                                                    
                                                                    
                                                                         
                                                                            
                                                                                Non-distribution item offered as a customer accommodation; additional freight charges may apply.
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| Shipping Condition | Dry Ice | 
|---|---|
| GC-Rich PCR Performance | Medium | 
| Fidelity (vs. Taq) | 1X | 
| Format | Tube | 
| Size (Final Product) | 5 kb or less | 
| Reaction Format | SuperMix or Master Mix | 
| Product Type | Hot Start PCR Master Mix | 
| Reaction Speed | Standard | 
| Hot Start | Built-In Hot Start | 
| Overhang | 3'-A | 
| Concentration | 2X | 
| Starting Material | DNA | 
| For Use With (Application) | Hot-start PCR | 
                                                                    Applied Biosystems™ TaqMan™ Rodent GAPDH Control Reagents
                                                            
                                                            
                                                            
                                                                
                                                            
                                                                
                                                            
                                        
                                                            
                                                            TaqMan™ Rodent GAPDH Control Reagents
 
                                                                            
                                                                                
                                                                            
                                                                    
                                                                    
                                                                         
                                                                            
                                                                                Non-distribution item offered as a customer accommodation; additional freight charges may apply.
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                                                                    Applied Biosystems™ Human PPIA (Cyclophilin A) Endogenous Control (VIC™/MGB probe, primer limited)
                                                            
                                                            
                                                            
                                                                
                                                            
                                                                        
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Human PPIA (Cyclophilin A) Endogenous Control (VIC™/MGB probe, primer limited)
 
                                                                            
                                                                                
                                                                            
                                                                    
                                                                    
                                                                         
                                                                            
                                                                                Non-distribution item offered as a customer accommodation; additional freight charges may apply.
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                                                                    Applied Biosystems™ Human GAPD (GAPDH) Endogenous Control (VIC™/MGB probe, primer limited)
                                                            
                                                            
                                                            
                                                                
                                                            
                                                                        
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Human GAPD (GAPDH) Endogenous Control (VIC™/MGB probe, primer limited)
 
                                                                            
                                                                                
                                                                            
                                                                    
                                                                    
                                                                         
                                                                            
                                                                                Non-distribution item offered as a customer accommodation; additional freight charges may apply.
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                                                                    Applied Biosystems™ Human B2M (Beta-2-Microglobulin) Endogenous Control (VIC™/MGB probe, primer limited)
                                                            
                                                            
                                                            
                                                                
                                                            
                                                                        
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Human B2M (Beta-2-Microglobulin) Endogenous Control (VIC™/MGB probe, primer limited)
 
                                                                            
                                                                                
                                                                            
                                                                    
                                                                    
                                                                         
                                                                            
                                                                                Non-distribution item offered as a customer accommodation; additional freight charges may apply.
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                                                                    Applied Biosystems™ Mouse ACTB (Actin, Beta) Endogenous Control (VIC™/MGB probe, primer limited)
                                                            
                                                            
                                                            
                                                                
                                                            
                                                                        
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                                                                                Greener Choice
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Mouse ACTB (Actin, Beta) Endogenous Control (VIC™/MGB probe, primer limited)
 
                                                                            
                                                                                
                                                                            
                                                                    
                                                                    
                                                                         
                                                                            
                                                                                Non-distribution item offered as a customer accommodation; additional freight charges may apply.
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                                                                    Applied Biosystems™ TaqMan™ Fast Reagent Starter Kit
                                                            
                                                            
                                                            
                                                                
                                                            
                                                                
                                                            
                                        
                                                            
                                                            Designed to detect and quantify Eukaryotic 18S rRNA sequence in cDNA samples using the TaqMan 5' nuclease assay, in fast thermal cycling mode.
 
                                                                            
                                                                                
                                                                            
                                                                    
                                                                    
                                                                         
                                                                            
                                                                                Non-distribution item offered as a customer accommodation; additional freight charges may apply.
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| Sample Type | DNA (Genomic), cDNA | 
|---|---|
| Reaction Speed | Fast | 
| For Use With (Application) | Gene Expression | 
| Detection Method | Primer-probe | 
                                                                    Invitrogen™ SuperScript™ IV Template Switching RT Master Mix
                                                            
                                                            
                                                            
                                                                
                                                            
                                                                
                                                            
                                        
                                                            
                                                            Invitrogen SuperScript IV Template-Switching RT Master Mix is designed for high template switching efficiency in cDNA synthesis reactions.
 
                                                                            
                                                                                
                                                                            
                                                                    
                                                                    
                                                                         
                                                                            
                                                                                Non-distribution item offered as a customer accommodation; additional freight charges may apply.
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| GC-Rich PCR Performance | High | 
|---|---|
| Format | Master Mix | 
| Final Product Type | First-Strand cDNA | 
| Reaction Speed | 30 min. | 
| Reagent Type | Reverse Transcription | 
| Technique | Template Switching Reverse Transcription | 
| Concentration | 4X | 
| Starting Material | Cells or RNA | 
                                                                    Applied Biosystems™ Eukaryotic 18S rRNA Endogenous Control (FAM™/MGB probe, non-primer limited)
                                                            
                                                            
                                                            
                                                                
                                                            
                                                                        
                                                                         Greener Choice
                                                                            
                                                                                Greener Choice
Offers one or more environmental benefits itemized in the U.S. FTC Green Guides.
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Eukaryotic 18S rRNA Endogenous Control (FAM™/MGB probe, non-primer limited)
 
                                                                            
                                                                                
                                                                            
                                                                    
                                                                    
                                                                         
                                                                            
                                                                                Non-distribution item offered as a customer accommodation; additional freight charges may apply.
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| Shipping Condition | Room Temperature | 
|---|---|
| Content And Storage | Control Primer-Probe Set(s) | 
| Gene Symbol | 18S | 
| Green Features | Sustainable packaging | 
| Amplicon Size | 187 | 
| Concentration | 20X | 
| For Use With (Application) | Gene Expression | 
| PCR Method | 2-step RT-qPCR | 
| Detection Method | Primer-probe | 
| Accession Number | X03205.1 | 
| Target | 18S rRNA | 
| Format | Tube | 
| Label or Dye | FAM | 
| Species | Eukaryotic, Rat, Mouse, Human | 
| Product Line | FAM™, TaqMan™ | 
                                                                    Applied Biosystems™ Human RPLPO (Large Ribosomal Protein) Endogenous Control (FAM™/MGB probe, non-primer limited)
                                                            
                                                            
                                                            
                                                                
                                                            
                                                                        
                                                                         Greener Choice
                                                                            
                                                                                Greener Choice
Offers one or more environmental benefits itemized in the U.S. FTC Green Guides.
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Human RPLPO (Large Ribosomal Protein) Endogenous Control (FAM™/MGB probe, non-primer limited)
 
                                                                            
                                                                                
                                                                            
                                                                    
                                                                    
                                                                         
                                                                            
                                                                                Non-distribution item offered as a customer accommodation; additional freight charges may apply.
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| Shipping Condition | Room Temperature | 
|---|---|
| Content And Storage | Control Primer-Probe Set(s) | 
| Gene Symbol | RPLPO | 
| Green Features | Sustainable packaging | 
| Amplicon Size | 105 | 
| Concentration | 20X | 
| For Use With (Application) | Gene Expression | 
| PCR Method | 2-step RT-qPCR | 
| Detection Method | Primer-probe | 
| Accession Number | NM_053275.3 | 
| Target | RPLPO | 
| Format | Tube | 
| Label or Dye | FAM | 
| Species | Human | 
| Product Line | FAM™, TaqMan™ | 
                                                                    Invitrogen™ SYBR™ Green Fast Advanced Cells-to-CT™ Kit
                                                            
                                                            
                                                            
                                                                
                                                            
                                                                        
                                                                         Greener Choice
                                                                            
                                                                                Greener Choice
Offers one or more environmental benefits itemized in the U.S. FTC Green Guides.
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The SYBR Green Fast Advanced Cells-to-Ct Kit is a complete cell lysate system designed for gene expression analysis directly from cultured cells without RNA purification.
 
                                                                            
                                                                                
                                                                            
                                                                    
                                                                    
                                                                         
                                                                            
                                                                                Non-distribution item offered as a customer accommodation; additional freight charges may apply.
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| Shipping Condition | Dry Ice | 
|---|---|
| GC-Rich PCR Performance | High | 
| Fidelity (vs. Taq) | 2X | 
| Green Features | Fewer resources used and less waste, Less hazardous | 
| Concentration | 2X | 
| For Use With (Application) | Gene Expression | 
| High-throughput Compatibility | High-throughput Compatible | 
| PCR Method | 2-step RT-qPCR | 
| Detection Method | SYBR | 
| Format | Tube | 
| Product Type | Fast Advanced Cells-to-Ct Kit | 
| Sample Type | Mammalian Cells | 
| Reaction Speed | 7 min. | 
| Reagent Type | Cell Lysis | 
| Purification Target | Lysate | 
| Starting Material | 10-100,000 Cells | 
| Product Line | Cells-to-CT™ | 
 
                                                             
                                                             
                                                             
                                                             
                                                             
                                                             
                                                             
                                                             
                                                            